Difference in Intestine Content of Caenorhabditis elegans When Fed on Non-Pathogenic or Pathogenic Bacteria

We investigated the bacterial food digestion and accumulation in wild-type adult Caenorhabditis elegans (C. elegans) worms that have fed on either non-pathogenic RFP-expressing Escherichia coli (E. coli) OP50 or pathogenic-RFP-expressing Pseudomonas aeruginosa (P. aeruginosa) PAO1 during first 4 days of adulthood. Once had completed their planned feeding cycles, they were loaded microfluidic chips, where fixed to allow high-resolution z-stack fluorescence imaging intestines utilizing a Spinning Disk Confocal Microscope (SDCM) equipped with oil-immersion objective (60×). IMARIS software was used visualize analyze obtained images, resulting production three-dimensional constructs intestinal load. discovered two distinct patterns for bacteria-derived signal intestine: (i) individual fluorescent spots, originating from intact bacteria, present E. coli-OP50-fed worms, (ii) spots (originating bacteria) dispersed large regions diffuse (RDF), disrupted P. aeruginosa-PAO1-fed worms. performed semi-automated single-worm-resolution quantitative analysis load, which showed load generally increases age but more rapidly